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The second response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as items (Fig. 9) - UV/Vis. As attracted Fig - https://visual.ly/users/julieanndesalorenz30606/portfolio. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As displayed in Fig. 9, this could be because the H4B extreme serve as an electron sink in a later point in the reaction, so regarding enable NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.